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Recombinant Enterokinas, CAS 9017-74-8, Enzyme, Enterokinase

Recombinant Enterokinas, CAS 9017-74-8, Enzyme, Enterokinase

Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: REK08
Minimum Order Quantity: 100U
Payment Terms: T/T
Place of Origin: China, Shanghai
Shanghai Yaxin Biotechnology Co.,Ltd.
Verified Supplier
Shanghai Shanghai China
Business type: Manufacturer Exporter Seller
No.466 YinDu Road,XuHui, Shanghai, China
Supplier`s last login times: within 33 hours

Recombinant Enterokinas, CAS 9017-74-8, Enzyme, Enterokinase


Description


YaxinBio Enterokinase is a kind of highly purified recombinant bovine enterokinase. The enzyme has been extensively purified and there are no traces of other contaminating proteases. Enterokinase specifically hydrolyzes peptide bond at the carboxyl side of lysine residue preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (D-D-D-D-K). So, Enterokinase can remove N-terminal fusion protein or tags to get aim protein with native amino acids sequence.


Advantages


1) Protease that cleaves specifically after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (D-D-D-D-K)

2) No any other contaminated proteases, no non-specific cutting sites.

Recommend Usage Condition:

Cutting condition: given an example: 25mM Tris-HCl 8.0

Fusion protein concentration: 0.1-1mg/ml (total protein content: 0.5-1.0mg)

EK content: 1-2U

Temperature: 25℃

Time: overnight or 16h-24h for digestion.


Main Features


M.W.

25,850 Da

Specific Activity

One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to16 hours to get 95% completion at 25°C in 25mMTris-HCl, pH 8.0. Substrate: a special fusion protein.

Storage Temperature

-20°C or below.

Stability

Keep cool with blue ice during shipping. Remained stable at 25°C for one week without activity lost. No activity lost after 5 cycles of frozen-thawing.


Common Components Influence the Action of Enterokinase


>200mM imidazole or >200mM NaCl or >5%glycerin, the reaction may be effected.The following suggestions are given:

1) To receive the optimum result, please dialyze the sample to 25 mMTris-HCl, pH 8.0.

2) If the dialysis is inconvenient, please dilute the sample to <100mM imidazole, <50mMNaCl, <5% glycerin, and the proportion of fusion protein and EK may not be changed (1U:0.5mg fusion protein).

3) If there are one or more components in samples, and cannot be removed, suggest to increase the content of EK in reaction system or extend the reaction time.


Product Information


Product

Activity

Packaging

Manufacturer

Recombinant Enterokinase

≥5 u/μl

100u;500u;1ku; 10ku

YaxinBio




Product Tags:

enterokinase enzyme

      

recombinant enzymes

      

Recombinant Enterokinas, CAS 9017-74-8, Enzyme, Enterokinase Images

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Recombinant Enterokinas, CAS 9017-74-8, Enzyme, Enterokinase