Recombinant Enterokinas, CAS 9017-74-8, Enzyme, Enterokinase
YaxinBio Enterokinase is a kind of highly purified recombinant
bovine enterokinase. The enzyme has been extensively purified and
there are no traces of other contaminating proteases. Enterokinase
specifically hydrolyzes peptide bond at the carboxyl side of lysine
residue preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys
(D-D-D-D-K). So, Enterokinase can remove N-terminal fusion protein
or tags to get aim protein with native amino acids sequence.
1) Protease that cleaves specifically after a lysine preceded by
four aspartic acids: Asp-Asp-Asp-Asp-Lys (D-D-D-D-K)
2) No any other contaminated proteases, no non-specific cutting
Recommend Usage Condition:
Cutting condition: given an example: 25mM Tris-HCl 8.0
Fusion protein concentration: 0.1-1mg/ml (total protein content:
EK content: 1-2U
Time: overnight or 16h-24h for digestion.
One unit is defined as the amount of enzyme needed to cleave 0.5mg
of fusion protein in 12 to16 hours to get 95% completion at 25°C in
25mMTris-HCl, pH 8.0. Substrate: a special fusion protein.
-20°C or below.
Keep cool with blue ice during shipping. Remained stable at 25°C
for one week without activity lost. No activity lost after 5 cycles
Common Components Influence the Action of Enterokinase
＞200mM imidazole or ＞200mM NaCl or ＞5%glycerin, the reaction may be
effected.The following suggestions are given:
1) To receive the optimum result, please dialyze the sample to 25
mMTris-HCl, pH 8.0.
2) If the dialysis is inconvenient, please dilute the sample to
＜100mM imidazole, ＜50mMNaCl, ＜5% glycerin, and the proportion of
fusion protein and EK may not be changed (1U:0.5mg fusion protein).
3) If there are one or more components in samples, and cannot be
removed, suggest to increase the content of EK in reaction system
or extend the reaction time.