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Bovine Light Chain Enterokinase, Recombinant Enterokinase, Expressed in E.coli

Bovine Light Chain Enterokinase, Recombinant Enterokinase, Expressed in E.coli

Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: REK08
Minimum Order Quantity: 100U
Payment Terms: T/T
Place of Origin: China, Shanghai
Shanghai Yaxin Biotechnology Co.,Ltd.
Verified Supplier
Shanghai Shanghai China
Business type: Manufacturer Exporter Seller
No.466 YinDu Road,XuHui, Shanghai, China
Supplier`s last login times: within 33 hours

Bovine Light Chain Enterokinase, Recombinant Enterokinase, Expressed in E.coli


Recombinant Enterokinase


Catalog Number:REK08

CAS:9017-74-8
EC:3.4.21.9

Source: bovine enterokinase, expressed in E. Coli


Description


YaxinBio Enterokinase is a kind of highly purified recombinant bovine enterokinase. The enzyme has been extensively purified and there are no traces of other contaminating proteases. Enterokinase specifically hydrolyzes peptide bond at the carboxyl side of lysine residue preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (D-D-D-D-K). So, Enterokinase can remove N-terminal fusion protein or tags to get aim protein with native amino acids sequence.


Product Overview


Recombinant Bovine Enterokinase produced in E.coli is a single glycosylated polypeptide chain containing 235 amino acids and having a molecular mass of approximately28 kDa.


Advantages


1) Protease that cleaves specifically after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (D-D-D-D-K)

2) No any other contaminated proteases, no non-specific cutting sites.


Recommend Usage Condition


Cutting conditiongiven an example: 25mM Tris-HCl 8.0
Fusion protein concentration0.1-1mg/ml (total protein content: 0.5-1.0mg)
EK content1-2U
Temperature25℃
Timeovernight or 16h-24h for digestion

Common Components Influence the Action of Enterokinase


>200mM imidazole or >200mM NaCl or >5%glycerin, the reaction may be effected.The following suggestions are given:

1) To receive the optimum result, please dialyze the sample to 25 mMTris-HCl, pH 8.0.

2) If the dialysis is inconvenient, please dilute the sample to <100mM imidazole, <50mMNaCl, <5% glycerin, and the proportion of fusion protein and EK may not be changed (1U:0.5mg fusion protein).

3) If there are one or more components in samples, and cannot be removed, suggest to increase the content of EK in reaction system or extend the reaction time.


Product Tags:

bovine enterokinase

      

enterokinase enzyme

      

Bovine Light Chain Enterokinase, Recombinant Enterokinase, Expressed in E.coli Images

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Bovine Light Chain Enterokinase, Recombinant Enterokinase, Expressed in E.coli