Bovine Light Chain Enterokinase, Recombinant Enterokinase, Expressed in E.coli

Bovine Light Chain Enterokinase, Recombinant Enterokinase, Expressed in E.coli

Recombinant Enterokinase

Catalog Number:REK08

CAS:9017-74-8
EC:3.4.21.9

Source: bovine enterokinase, expressed in E. Coli

Description

YaxinBio Enterokinase is a kind of highly purified recombinant bovine enterokinase. The enzyme has been extensively purified and there are no traces of other contaminating proteases. Enterokinase specifically hydrolyzes peptide bond at the carboxyl side of lysine residue preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (D-D-D-D-K). So, Enterokinase can remove N-terminal fusion protein or tags to get aim protein with native amino acids sequence.

Product Overview

Recombinant Bovine Enterokinase produced in E.coli is a single glycosylated polypeptide chain containing 235 amino acids and having a molecular mass of approximately28 kDa.

Advantages

1) Protease that cleaves specifically after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (D-D-D-D-K)

2) No any other contaminated proteases, no non-specific cutting sites.

Recommend Usage Condition

Common Components Influence the Action of Enterokinase

＞200mM imidazole or ＞200mM NaCl or ＞5%glycerin, the reaction may be effected.The following suggestions are given:

1) To receive the optimum result, please dialyze the sample to 25 mMTris-HCl, pH 8.0.

2) If the dialysis is inconvenient, please dilute the sample to ＜100mM imidazole, ＜50mMNaCl, ＜5% glycerin, and the proportion of fusion protein and EK may not be changed (1U:0.5mg fusion protein).

3) If there are one or more components in samples, and cannot be removed, suggest to increase the content of EK in reaction system or extend the reaction time.