登录 | 免费注册 | 提交网站 | 免费看询盘 | 一站通免费建站 | "贸易通"多语言推广 | 外贸论坛
Hot Products:
Home > Bamboo Raw Materials >

Removing Unwanted Protein Tags Recombinant Bovine Enterokinase REK

Removing Unwanted Protein Tags Recombinant Bovine Enterokinase REK

Brand Name: YaxinBio
Certification: NSF ISO 9001-2008
Model Number: REK08
Minimum Order Quantity: 100U
Payment Terms: T/T
Place of Origin: China
Shanghai Yaxin Biotechnology Co.,Ltd.
Verified Supplier
Shanghai Shanghai China
Business type: Manufacturer Exporter Seller
Telephone :
No.466 YinDu Road,XuHui, Shanghai, China
Supplier`s last login times: within 33 hours
English

Removing Unwanted Protein Tags Recombinant Bovine Enterokinase REK


Description


YaxinBio Enterokinase is a kind of highly purified recombinant bovine enterokinase. The enzyme has been extensively purified and there are no traces of other contaminating proteases. Enterokinase specifically hydrolyzes peptide bond at the carboxyl side of lysine residue preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (DDDDK). So, Enterokinase can remove N-terminal fusion protein or tags to get aim protein with native amino acids sequence.

CAS:9017-74-8
EC:3.4.21.9

Source: bovine enterokinase, expressed in E. Coli


Common components influence the action of enterokinase

>200mM imidazole or >200mM NaCl or >5%glycerin, the reaction may be effected.The following suggestions are given:

1) To receive the optimum result, please dialyze the sample to 25 mMTris-HCl, pH 8.0.

2) If the dialysis is inconvenient, please dilute the sample to <100mM imidazole, <50mMNaCl, <5% glycerin, and the proportion of fusion protein and EK may not be changed (1U:0.5mg fusion protein).

3) If there are one or more components in samples, and cannot be removed, suggest to increase the content of EK in reaction system or extend the reaction time.


Main Features Advantages


M.W.

25,850 Da

Specific Activity

One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to16 hours to get 95% completion at 25°C in 25mMTris-HCl, pH 8.0. Substrate: a special fusion protein.

Storage Temperature

-20°C or below.

Stability

Keep cool with blue ice during shipping. Remained stable at 25°C for one week without activity lost. No activity lost after 5 cycles of frozen-thawing.


Application


Enterokinase is a member of the S1 peptidase family. In vivo, it is responsble for the proteolytic activation of trypsin from trypsinogen. Enterokinase is used for site specific cleavage of recombinant fusion proteins containing an accessible enterokinase recognition site for removal of affinity tags.

Enterokinase from bovine intestine has been used in a study to assess duodenase as a potential activator of cascade digestive proteases. Enterokinase from bovine intestine has also been used in a study to investigate an inhibitor of enteropeptidases and trypsin from the bovine duodenum.

The enzyme has been used to compare the specific activity with that of purified, recombinant bovine enterokinase (light chain) overexpressed inEscherichia coli.


Email:Zhaozhi@yaxinbio.com


Product Tags:

bovine enterokinase

      

recombinant enzymes

      

Removing Unwanted Protein Tags Recombinant Bovine Enterokinase REK Images

Send your message to this supplier
From:
Enter your email please.
To: Shanghai Yaxin Biotechnology Co.,Ltd.
Subject:
Message:
Characters Remaining: (0/3000)
 
Inquiry Cart 0
Other about

Removing Unwanted Protein Tags Recombinant Bovine Enterokinase REK

Bovine Light Chain Enterokinase Recombinant Enterokinase E.coli

Brand Name :YaxinBio
Certification :ISO 9001-2008

Recombinant Enterokinase Enzyme and CAS 9017-74-8 Enzyme Enterokinase

Brand Name :YaxinBio
Certification :NSF ISO 9001-2008