Recombinant Human Trypsin Solution Animal Origin Free Human Trypsin
Trypsin is a member of the serine protease family. Trypsin cleaves
peptides on the C-terminal end of lysine and arginine amino acid
residues. The optimum pH is pH 7.0 - 8.0. The enzyme is inhibited
by serine protease inhibitors, e.g. PMSF, and by metal chelating
agents, e.g., EDTA.
Recombinant human trypsin is a genetically engineered protein
expressed in E.coli and purified by high pressure liquid chromatography. There are no
contaminating enzyme activities such as carboxypeptidase A and
chymotrypsin. No protease inhibitors such as PMSF are contained in
TrypZean eliminates the risk of viruses, BSE, or other potential
TrypZean is recombinant trypsin
Using the same enzyme (with the same kinetics) for cell detachment
means minimal protocol changes for you.
Soybean trypsin inhibitor and other inhibitors work the same with
TrypZean as they do with native trypsins (on a weight-to-weight
TrypZean provides increased specificity and eliminates
contaminating activities found in lower purity enzymes.
TrypZean Solution is formulated at the optimal concentration to
dissociate adherent cells.
White or White-like lyophilized
≥ 95% by HPLC
No chymotrypsin, carboxypeptidase A, and other protease
Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003
per minute in a reaction volume of 3.0ml at pH7.6 and 25℃, with
BAEE as a substrate (1cm light path).
Recombinant human trypsin lyophilized should be
stored under 2℃-8℃ in sealed container. It is stable within 24
months.After dissolved, it should be stored under -20℃. It is
stable within 24 months and above 90% activity
remained after 10 times repeated freezing and thawing.