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Recombinant Human Trypsin Solution Animal Origin Free Human Trypsin

Recombinant Human Trypsin Solution Animal Origin Free Human Trypsin

Brand Name: YaxinBio
Certification: ISO 9001-2008
Model Number: RHT03
Minimum Order Quantity: 10mg
Delivery Time: Six days
Payment Terms: T/T
Shanghai Yaxin Biotechnology Co.,Ltd.
Verified Supplier
Shanghai Shanghai China
Business type: Manufacturer Exporter Seller
Telephone :
No.466 YinDu Road,XuHui, Shanghai, China
Supplier`s last login times: within 33 hours
English

Recombinant Human Trypsin Solution Animal Origin Free Human Trypsin


Deacription


Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH is pH 7.0 - 8.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA.

Recombinant human trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.


Advantages


Animal component-free
TrypZean eliminates the risk of viruses, BSE, or other potential adventitious agents.

TrypZean is recombinant trypsin
Using the same enzyme (with the same kinetics) for cell detachment means minimal protocol changes for you.

Enzyme inhibition
Soybean trypsin inhibitor and other inhibitors work the same with TrypZean as they do with native trypsins (on a weight-to-weight basis).

High purity
TrypZean provides increased specificity and eliminates contaminating activities found in lower purity enzymes.

Convenience
TrypZean Solution is formulated at the optimal concentration to dissociate adherent cells.


Main Features


Source

E. Coli

Purified by

HPLC

Format

White or White-like lyophilized

Purity

≥ 95% by HPLC

Contaminant activity

No chymotrypsin, carboxypeptidase A, and other protease contaminant.


Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).


Storage Instructions


Recombinant human trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.


Email:Zhaozhi@yaxinbio.com



Product Tags:

trypsinized cells

      

porcine trypsin

      

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