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Cell Dissociation Solution, Recombinant Trypsin, Expressed in E.coli, For Cell Fermentation

Cell Dissociation Solution, Recombinant Trypsin, Expressed in E.coli, For Cell Fermentation

Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RPT0201
Minimum Order Quantity: 10mg
Payment Terms: T/T
Place of Origin: China, Shanghai
Shanghai Yaxin Biotechnology Co.,Ltd.
Verified Supplier
Shanghai Shanghai China
Business type: Manufacturer Exporter Seller
No.466 YinDu Road,XuHui, Shanghai, China
Supplier`s last login times: within 33 hours

Cell Dissociation Solution, Recombinant Trypsin, Expressed in E.coli, For Cell Fermentation


Recombinant Trypsin


Description


Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF and by metal chelating agents, e.g.EDTA.

Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.


Main Features


Defined, animal component-free

Ready-to-use solution

Pure, recombinant trypsin - no contaminating proteases
Optimized for sensitive cells, such as hMSCs
Compatible with cells cultured in both serum-free and serum-containing media


Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).


Recommend Usage


Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7-10.


Storage Instructions


Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.


Application


Recombinant trypsin solution is a pure enzyme solution, helps to maximize the yield of functionally viable cells from culture vessels, while preventing the toxicity effect induced by other non desirable proteases. In addition, Rec. Trypsin eliminates the risk of viruses, or other potential adventitious agents found in animal derived solutions. This special animal component free, recombinant Trypsin solution, is an alternative for bulk production of porcine trypsin . Rec.Trypsin is produced by submerged fermentation of Fusarium oxysporum. It is derived from a production process which does not utilize any raw materials and/or processing aids of animal origin.



Product Tags:

dissociated cell culture

      

lyophilized powder

      

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Cell Dissociation Solution, Recombinant Trypsin, Expressed in E.coli, For Cell Fermentatio