登录 | 免费注册 | 提交网站 | 免费看询盘 | 外贸旺站免费建站 | "贸易通"多语言推广 | 外贸论坛
Hot Products:
Home > Cardiovascular Agents >

Human Trypsin, Cleave C-terminal Peptide Bond of Lysine and Arginine

Human Trypsin, Cleave C-terminal Peptide Bond of Lysine and Arginine

Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RHT03
Minimum Order Quantity: 10mg
Delivery Time: 5 days
Payment Terms: T/T
Shanghai Yaxin Biotechnology Co.,Ltd.
Verified Supplier
Shanghai Shanghai China
Business type: Manufacturer Exporter Seller
No.466 YinDu Road,XuHui, Shanghai, China
Supplier`s last login times: within 33 hours

Human Trypsin, Cleave C-terminal Peptide Bond of Lysine and Arginine


Human Trypsin


Description


Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA.

Recombinant Human Trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.


Advantages


Animal origin free:The use of recombinant Human Trypsin eliminates the risk of virus presence, and of any other potential adventitious agents found in animal pancreas-derived trypsin. YaxinBio Recombinant Human Trypsin belongs to the AOF level 3.

Recombinant human trypsin:The amino acid sequence is the same as the Human Trypsin 2.

Stable:A sterile recombinant human trypsin lyophilized eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

High purity:

1) Recombinant human trypsin provides increased specificity and eliminates contaminating activities found in lower purity enzymes.

2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.

3) Purity is more than 95% by HPLC.


Unit Definition


One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).


RECOMMEND USAGE

Prepare 1-10mg/ml recombinant human trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.



Product Tags:

stem cell culture

      

trypsinized cells

      

Human Trypsin, Cleave C-terminal Peptide Bond of Lysine and Arginine Images

Send your message to this supplier
From:
Enter your email please.
To: Shanghai Yaxin Biotechnology Co.,Ltd.
Subject:
Message:
Characters Remaining: (0/3000)
 
Inquiry Cart 0
Other about

Human Trypsin, Cleave C-terminal Peptide Bond of Lysine and Arginine