Carboxypeptidase B, Sequencing Grade Carboxypeptidase B, For Mass
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids
lysine, arginine and histidine from the C-terminal end of
polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the
optimum pH is 7.5-9.0. Carboxypeptidase B is competitively
inhibited by arginine and lysine. The enzyme is also inhibited by
metal chelating agents, e.g., EDTA.
2. MAIN FEATURES
|Source||Recombinant E. coli|
|Appearance||White or off white, or yellowish powder|
|Specific activity||≥200 units/mg pro.|
|SDS-PAGE||Single major band|
|Molecular Weight(SDS-PAGE)||33.8±3.4 kDa|
Formulation:The protein was lyophilized with 100mM NaCl and 20mM
Solubility:It is recommended to reconstitute the lyophilized Rat
Carboxypeptidase-B in sterile
18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to
other aqueous solutions.
Purity: Greater than 95% as determined by SDS-PAGE.
Unit Definition: One Unit hydrolyzes one micromole of
hippuryl-L-arginine per minute at 25°C, pH-7.65.
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY.
The product may
not be used as drugs, agricultural or pesticidal products, food
additives or household chemicals.
Recombinant Rat Carboxypeptidase-B although stable at room temp for
1 week, should be stored desiccated below 2-8°C. For long term
storage it is recommended to be stored below -20℃.Please prevent