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Carboxypeptidase B, Sequencing Grade Carboxypeptidase B, For Mass Spectrometry

Carboxypeptidase B, Sequencing Grade Carboxypeptidase B, For Mass Spectrometry

Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: SRCPB01
Minimum Order Quantity: 100ug
Payment Terms: T/T
Place of Origin: China, Shanghai
Shanghai Yaxin Biotechnology Co.,Ltd.
Verified Supplier
Shanghai Shanghai China
Business type: Manufacturer Exporter Seller
No.466 YinDu Road,XuHui, Shanghai, China
Supplier`s last login times: within 33 hours

Carboxypeptidase B, Sequencing Grade Carboxypeptidase B, For Mass Spectrometry


1.DESCRIPTION

Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA.


2. MAIN FEATURES

SourceRecombinant E. coli
AppearanceWhite or off white, or yellowish powder
Specific activity≥200 units/mg pro.
SDS-PAGESingle major band
Molecular Weight(SDS-PAGE)33.8±3.4 kDa
Purity(HPLC)≥ 95%

Formulation:The protein was lyophilized with 100mM NaCl and 20mM Tris pH-7.5.

Solubility:It is recommended to reconstitute the lyophilized Rat Carboxypeptidase-B in sterile

18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Purity: Greater than 95% as determined by SDS-PAGE.

Unit Definition: One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C, pH-7.65.


Usage


ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may

not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


Stability


Recombinant Rat Carboxypeptidase-B although stable at room temp for 1 week, should be stored desiccated below 2-8°C. For long term storage it is recommended to be stored below -20℃.Please prevent freeze-thaw cycles.


Product Tags:

trypsin endopeptidase

      

carboxypeptidase enzyme

      

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Carboxypeptidase B, Sequencing Grade Carboxypeptidase B, For Mass Spectrometry