Sequencing Grade Modified Trypsin, Used for Peptide Fingerprinting
Analysis CAS 9002-07-7
Sequencing Grade Trypsin
Source:Expressed in E. Coli, methylation modified.
Trypsin specifically hydrolyzes peptide bonds at the carboxyl side
of lysine and arginine residues. Recombinant trypsin is free of any
other proteases activities, and TPCK is unnecessary and not
contained. Unmodified trypsin is subject to auto-proteolysis,
generating fragments that can interfere with protein sequencing or
HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified
trypsin is recombinant porcine trypsin modified by reductive
methylation, rendering it resistant to proteolytic digestion.
One USP unit of trypsin activity will produce a Delta A253 of 0.003
per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with
BAEE as a substrate (1cm light path).
Protein digests for peptide mapping applications or protein
identification by peptide mass fingerprinting or MS/MS spectral
matching. It is suitable for digestion reactions in-solution or
1.Dilute recombinant modified trypsin with 50mM HAc if needed. When
used, dilute it with 50mM NH4HCO3 or pH7-8 buffers directly. 1mM CaCl2 is recommended to be contained in digestion buffer. The ratio to
aimed protein is 1:20 to 1:100 (w/w), the optimum pH is pH7-8.
2. When using this product, thaw it at room temperature, and mix
gently before use.
3. No activity lost when freeze-thaw 5 cycles.
1. The solution should be stored under -70℃, It is stable within 24
2. Above 95% activity remained after 5 times repeated freezing and
3. A 0.05 mg/ml solution of sequencing grade modified recombinant
trypsin retained above 95% after a 3 hours incubation at 37 ℃ in
50mM NH4HCO3. For long-term such as 20hours incubation, 1mM CaCl2 is recommended to be contained.
Following reconstitution in 10 mM HCl, Trypsin, recombinant,
proteomics grade is stable at -15 to -25 °C for up to one month.
After first reconstitution the product must be stored in
appropriate aliquots to avoid repeated freezing and thawing.
Serine endopeptidase, hydrolyzing specifically proteins and
peptides at the carboxy side of the basic amino acids Arg and Lys.
Amide and ester bonds of Arg and Lys are also cleaved.
Trypsin recombinant, Proteomics Grade, is used to specifically
digest proteins separated by 2D gel electrophoresis or liquid
chromatographic methods during sample preparation for mass