Carboxypeptidase B Sequence Grade For Mass Spectrometry Analysising
lysine Peak Antibody
Source:Rat carboxypeptidase B, expressed in E. Coli.
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids
lysine, arginine and histidine from the C-terminal end of
polypeptides. The molecular weight is 33.8kD, the pH optimum is
8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited
by arginine and lysine. The enzyme is also inhibited by metal
chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is
expressed in E.Coli and purified by high pressure liquid
chromatography. There is no trace of other enzyme (such as
carboxypeptidase A and chymotrypsin) activity. No protease
inhibitors such as PMSF are present in the preparation.
Protein structure and sequence analysis, such as hydrolyze basic
amino acids lysine, arginine and histidine from the C-terminal end
Antibody quality control.
Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM
Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000
(w/w), the optimum pH is pH 7-9.
Recommend recombinant carboxypeptidase B lyophilized should be
stored under 2-8℃ in sealed container. It is stable within 24
months.After dissolved, it should be stored under -20℃, It is
stable within 24 months and no activity lose after 10 times
repeated freezing and thawing.