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Recombinant Trypsin E.coli Animal Components Free Trypsin

Recombinant Trypsin E.coli Animal Components Free Trypsin

Brand Name: YaxinBio
Certification: ISO9001
Packaging: ice bag
Delivery Time: 7 days
Payment Terms: T/T
Shanghai Yaxin Biotechnology Co.,Ltd.
Verified Supplier
Shanghai Shanghai China
Business type: Manufacturer Exporter Seller
Telephone : 86-21-54336592
No.466 YinDu Road,XuHui, Shanghai, China
Supplier`s last login times: within 33 hours
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Recombinant Trypsin E.coli Animal Components Free Trypsin

 

 

Description

 

Trypsin
Enzyme Commission (EC) Number: 3.4.21.4
Molecular Weight: 23.3 kDa (porcine)
Extinction Coefficient: E1% = 12.9 - 15.4 (280 nm)
pI: 10.1 - 10.5(porcine)

Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The pH optimum of trypsin is pH 7.0 - 9.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF, aprotinin, soybean trypsin inhibitor and by metal chelating agents, e.g., EDTA.

Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified by

high pressure liquid chromatography. There are no any other contaminating enzyme activities. No protease inhibitors such as PMSF are contained in the preparation.
Trypsinogen
Molecular Weight: 24 kDa5 (porcine)
Extinction Coefficient: E1% = 14.4 (280 nm)
pI: 9.32 (porcine)

Trypsinogen, the proenzyme (zymogen) form of trypsin, is produced in the acinar exocrine cells of the pancreas. Three isoforms are excreted from the human pancreas. The cationic and anionic forms are the predominant human isoforms. The inhibitor-resistant mesotrypsinogen is found only in trace amounts.The proenzyme is activated only after it reaches the lumen of the small intestine. Enterokinase activates pancreatic trypsinogen to trypsin by the hydrolysis of a hexapeptide(for bovine trypsin at the Lys - Ile peptide bond) from the NH2 terminus.

 

Advantages

 

Animal origin free:The use of recombinant porcine trypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant Porcine Trypsin belongs to the AOF level 3.

Stablility: A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

High purity:Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.No other contaminating proteases such as chymotrypsin or carboxypeptidase A.

 

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Recombinant Trypsin E.coli Animal Components Free Trypsin

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